Figure 3: ATO induced expression of SIRPα protein and apoptosis in APL cell lines but not in hepatocellular carcinoma cell line. | Scientific Reports

Figure 3: ATO induced expression of SIRPα protein and apoptosis in APL cell lines but not in hepatocellular carcinoma cell line.

From: Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis

Figure 3

(a) Western blotting of SIRPα level in HL-60, NB4 and Huh7 cells treated with ATO for indicated time, the THP-1 whole cell lysate was used as a positive control: representative Western blotting (left panel) and quantitative analysis of SIRPα level (right panel). (b) Immunofluorescence analysis of SIRPα protein induced in HL-60, NB4 and Huh7 cells with ATO treatment for 24 h. (c) Cleaved caspase-3 level in HL-60, NB4 and Huh7 cells treated with ATO at indicated time: representative Western blot (left panel) and quantitative analysis (right panel). (d) Flow cytometry analysis of ATO-treated HL-60, NB4 and Huh7 cells for indicated time with annexin V-PI staining: representative flow cytometer data (left panel) and quantitative analysis of apoptosis (right panel). The percentage of annexin V positive cells was calculated. Values were shown as the mean ± SEM (n = 3). *P < 0.05. **P < 0.01.

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