Figure 4: Block of SIRPα induction attenuated ATO-induced apoptosis of APL cell lines. | Scientific Reports

Figure 4: Block of SIRPα induction attenuated ATO-induced apoptosis of APL cell lines.

From: Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis

Figure 4

SIRPα and cleaved caspase-3 protein level in SIRPα shRNA lentivirus-infected HL-60 or NB4 cells treated with ATO for indicated time: representative Western blots (a) and quantitative analysis of Western blot (b). Cells treated without lentivirus (PBS) or the cells infected with CTL shRNA lentivirus were used as controls. (c) Flow cytometry analysis of annexin V-PI staining in SIRPα shRNA lentivirus-infected HL-60 or NB4 cells in the presence of ATO for indicated time. Left panel, representative flow cytometer data. Right panel, quantitative analysis of apoptosis. Values were shown as the mean ± SEM (n = 3). *P < 0.05. **P < 0.01.

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