Figure 5: Induction of SIRPα contributed to the downregulation of β-catenin and upregulation of Foxo3a in APL cell lines treated with ATO. | Scientific Reports

Figure 5: Induction of SIRPα contributed to the downregulation of β-catenin and upregulation of Foxo3a in APL cell lines treated with ATO.

From: Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis

Figure 5

(a,b) Western blotting of p-Akt, Akt, p-GSK-3β, GSK-3β, β-catenin, Foxo3a and GAPDH in HL-60 or NB4 cells treated with ATO for indicated time: representative Western blotting (a) and quantitative analysis of protein level (b), the protein level of GAPDH was used as an internal control, the p-Akt (Ser473) and p-GSK-3β (Ser9) were normalized to the total Akt and GSK-3β, respectively. (c,d) Western blotting of p-Akt, Akt, p-GSK-3β, GSK-3β, β-catenin, Foxo3a and GAPDH in SIRPα shRNA lentivirus infected HL-60 or NB4 cells after the treatment of ATO for 48 h: representative Western blot (c) and quantitative analysis (d). Values were shown as the mean ± SEM (n = 3). *P < 0.05. **P < 0.01.

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