Figure 7: ATO suppressed miR-17, miR20a, and miR-106a through the inhibition of c-Myc expression. | Scientific Reports

Figure 7: ATO suppressed miR-17, miR20a, and miR-106a through the inhibition of c-Myc expression.

From: Role of Signal Regulatory Protein α in Arsenic Trioxide-induced Promyelocytic Leukemia Cell Apoptosis

Figure 7

(a) Western blotting analysis of c-Myc level in HL-60 or NB4 cells treated with ATO for indicated time: representative Western blot (upper panel) and quantitative analysis (lower panel). (b) Western blotting of c-Myc and SIRPα in c-Myc overexpression lentivirus (LV-c-Myc) infected HL-60 or NB4 cells after treated with ATO for 48 hours. The lentivirus (LV-CTL) was used as a control: representative Western blotting (upper panel) and quantitative analysis (lower panel). (c) The level of SIRPα-regulating miRNAs, miR-17, miR-20a, miR-106a in LV-c-Myc infected HL-60 or NB4 cells after treated with ATO for 48 h, LV-CTL was used as a control. (d) Flow cytometry analysis of apoptosis of LV-c-Myc infected HL-60 or NB4 cells after the treatment of ATO for 48 h: representative flow cytometer results (left panel) and quantitative analysis of apoptosis (right panel). The percentage of annexin V-positive cells was calculated. Values were shown as the mean ± SEM (n = 3). *P < 0.05. **P < 0.01.

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