Figure 3

Analysis of the dimerization of UGT1As by Co-IP.
The HA-tagged and CFP-tagged proteins were directly mixed and no CFP-tagged protein bands were observed after Co-IP (A,B). The HA- and CFP-tagged proteins were co-expressed in host cells followed by anti-HA beads immunoprecipitation and western blotting detection, wherein two bands (56 kDa and 81 kDa) were observed (C,D).