Table 1 Apparent FRET efficiency values E and donor-acceptor distances r of co-expression proteins.
protein-protein | FRET efficiency E % | donor-acceptor distance r (nm) | n |
|---|---|---|---|
CFP/YFP | −1.56 ± 4.8 | >10 | 5 |
CFP-linker-YFP | 17.4 ± 4.8 | 6.92 | 21 |
1A1*1-CFP/1A1*1-YFP | 18.3 ± 4.9 | 6.77 | 7 |
1A1*1b-CFP/1A1*1b-YFP | 23.6 ± 7.8 | 6.41 | 13 |
1A1*1-CFP/1A1*1b-YFP | 14.5 ± 3.4 | 7.09 | 19 |
1A9*1-CFP/1A9*1-YFP | 25.1 ± 11.5 | 6.33 | 27 |
1A9*2-CFP/1A9*2-YFP | 21.9 ± 3.8 | 6.52 | 10 |
1A9*3-CFP/1A9*3-YFP | 13.8 ± 6.5 | 7.16 | 8 |
1A9*5-CFP/1A9*5-YFP | 9.6 ± 4.1 | 7.67 | 18 |
1A9*1-CFP/1A9*2-YFP | 11.2 ± 4.3 | 7.45 | 16 |
1A9*1-CFP/1A9*3-YFP | 19.9 ± 8.6 | 6.65 | 20 |
1A9*1-CFP/1A9*5-YFP | 17.0 ± 8.9 | 6.87 | 16 |
1A9*2-CFP/1A9*3-YFP | 12.3 ± 4.7 | 7.32 | 15 |
1A9*2-CFP/1A9*5-YFP | 9.9 ± 5.8 | 7.62 | 12 |
1A9*3-CFP/1A9*5-YFP | 15.5 ± 6.7 | 7.00 | 11 |
1A9*1-CFP/1A9*1-YFP (+1% Methanol) | 17.2 ± 8.2 | 6.86 | 20 |
1A9*1-CFP/1A9*1-YFP (+150 μmol/L quercetin) | 7.84 ± 3.0 | 7.96 | 10 |
1A9*1-CFP/1A9*1-YFP (+5 mmol/L UDPGA) | 22.9 ± 9.9 | 6.46 | 13 |
