Table 3 Kinetic parameters for quercetin-7-glucuronide by UGT1A9 double expression systems (n = 3).

1A9N-1A9N	K_m (μmol/L)	K_si (μmol/L)	V_max (pmol/min/unit)	CL_int (μL/min/unit)	% of 1A9*1-HA	% of 1A9*1-CFP
1A91-CFP + 1A92-HA	14.43 ± 1.09		519.0 ± 9.7	35.97^**	43.55	33.37
1A91-CFP + 1A93-HA	39.86 ± 9.74	254.0 ± 107.9	2977 ± 448.6	74.69^*	90.42	69.29
1A91-CFP + 1A95-HA	31.97 ± 8.93	210.0 ± 91.6	1040 ± 173.0	32.53	39.38	30.18
1A92-CFP + 1A93-HA	34.33 ± 5.82		675.0 ± 39.8	19.66^**	23.80	18.24
1A92-CFP + 1A95-HA	12.92 ± 1.24		145.7 ± 3.6	11.27^***	13.64	10.45
1A93-CFP + 1A95-HA	34.66 ± 2.73		514.2 ± 14.2	14.8^**	17.92	13.73

^*reprensts the CL_int of double expression compared with that of the single expression with the two single expression plus together in the bracket. Data are the mean ± SD of three independent determinations and the asterisks indicate differences that are statistically significant (^***P < 0.001, ^**P < 0.005, ^*P < 0.05).

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