Figure 7 | Scientific Reports

Figure 7

From: Genome-wide generation and use of informative intron-spanning and intron-length polymorphism markers for high-throughput genetic analysis in rice

Figure 7

Schematic depicting the key steps followed for successful discovery, large-scale validation and high-throughput genotyping of ISM and ILP markers derived from diverse intronic sequence components of grain weight-regulating known cloned gene as exemplified by GW8/SPL16 (annotated from rice genome), to be utilized for multi-dimensional genomics-assisted breeding applications in rice.

The Forward (F) and Reverse (R) primers designed from the exonic sequences flanking the introns (without any InDels) and intronic-InDels were developed as ISM and ILP markers, respectively. URR: Upstream regulatory region and DRR: downstream regulatory region. The identities of ISM and ILP markers with their detailed information are mentioned in the Tables S1 and S2.

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