Figure 2 | Scientific Reports

Figure 2

From: BET Bromodomain Suppression Inhibits VEGF-induced Angiogenesis and Vascular Permeability by Blocking VEGFR2-mediated Activation of PAK1 and eNOS

Figure 2

Effect of the BET bromodomain inhibitor JQ1 on vascular hyperpermeability.

(A) Electrical resistance of a monolayer of HUVECs was measured by TEER. The cells were cultured to confluence in 24-well Transwell clear polyester membrane cell culture chambers, treated with various concentration of JQ1 or 0.1% DMSO for 6 h and then stimulated with VEGF (10 ng/mL) or PBS for 4 h or 16 h. The experiments were carried out in triplicate in 3 separate experiments. (B,C) The BET inhibitor JQ1 suppressed VEGF-induced vascular permeability in the Miles assay in athymic Balb/c nude mice. Mice were pretreated intradermally with DMSO (as a control) or JQ1 (50 mg/kg·d) for 5 d. VEGF (100 ng in 50 μL/site) or PBS was injected intradermally in the dorsal skin and the ear. Mice were killed and skin samples (B) and ears (C) were dissected, photographed and placed in formamide at 56 °C for 48 h to extract Evans blue. The amount of Evans blue in each sample was read by spectrophotometry. All values represent mean ± S.E.M. *P < 0.05 vs. control, #P < 0.05 vs. VEGF.

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