Figure 3
Effect of BET bromodomain inhibition on migration, cytoskeleton alterations and proliferation of HUVECs.
(A,B) Migration was performed in a Boyden chamber and chemotaxis was quantified by counting migrated cells. Photo images of migration of HUVECs pretreated with various concentrations of JQ1 (A) or transfected with Brd2 shRNA or Brd4 shRNA or control shRNA (shC) (B) and then stimulated with VEGF (10 ng/mL) or DMSO. (C) Effect of JQ1 on wound migration of HUVECs induced by VEGF (original magnification ×100). Cells migrating beyond the reference line were photographed and counted. (D) Effect of JQ1 on alterations of actin cytoskeleton in VEGF-induced HUVECs. The cells were pretreated with DMSO or JQ1 (100 nM) for 6 h and stimulated with VEGF (10 ng/mL) for 12 h. F-actin (red) and nuclei (blue) were stained with phalloidin and DAPI, respectively. Representative images from 3 independent experiments. (E) Effect of JQ1 on proliferation of HUVECs. The cells were pretreated with DMSO or various concentration of JQ1 and then stimulated with VEGF (10 ng/mL) for 24 h. EdU incorporation was used to assess proliferation of cells. Representative images from 3 independent experiments. All values represent mean ± SD. *P < 0.05 vs. control, #P < 0.05 vs. VEGF.
