Figure 5

The role of PDCD4 targeting through miR-93 in the regulation of apoptosis in gastric cancer cells.

AGS cells were transfected with equal doses of pre-miR-control, pre-miR-93, anti-miR-control, anti-miR-93, control siRNA, PDCD4 siRNA, control plasmid, PDCD4 plasmid, pre-miR-control plus control plasmid, pre-miR-93 plus control plasmid, pre-miR-control plus PDCD4 plasmid, or pre-miR-93 plus PDCD4 plasmid. (A,B) The cell apoptosis profiles were analyzed using Annexin V-FITC/PI staining in flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (upper right quadrant). Viable cells are double negative (bottom left quadrant). (A) representative image; (B) quantitative analysis. (C) Representative images of apoptotic AGS cells analyzed using DAPI staining. DAPI staining is used to monitor the apoptosis of cells by exhibiting a strong fluorescence when DAPI becomes bound to natural double-stranded DNA. The normal cell nuclei are round in shape and staining is evenly distributed. When the cells become apoptotic, the cell nuclei become deformed due to the aggregation of the DNA. (D) Representative images of apoptotic AGS cells analyzed using TUNEL staining. TUNEL staining is used to monitor the apoptosis of cells by exhibiting a strong fluorescence when DNA strand breaks in the apoptotic cells and is labeled with fluorescein-12-dUTP. *P < 0.05; **P < 0.01.