Figure 6

Effects of miR-93 and PDCD4 on the growth of gastric cancer cell xenografts in mice.

AGS cells were infected with a control lentivirus or a miR-93 overexpression lentivirus, or transfected with a PDCD4 overexpression plasmid, or co-transfected with the miR-93 overexpression lentivirus plus PDCD4 overexpression plasmid. Then the cells (2 × 10⁶ cells per 0.1 mL) were implanted subcutaneously into 6-week-old SCID mice (5 mice per group) and tumor growth was evaluated at day 60 after cell implantation. (A) Representative images of the tumors from the implanted mice. (B) Quantitative analysis of the tumor weights. (C) Quantitative RT-PCR analysis of miR-93 levels in the tumors from implanted mice. (D) Quantitative RT-PCR analysis of PDCD4 mRNA levels in the tumors from implanted mice. (E,F) Western blotting analysis of PDCD4 protein levels in the tumors from implanted mice. (E) representative image; (F) quantitative analysis. (G–I) H & E-stained sections and immunohistochemical staining for PDCD4 and Ki-67 in the tumors from implanted mice. (G) representative image; (H,I) quantitative analysis. **p < 0.01; ***p < 0.001.