Figure 10 | Scientific Reports

Figure 10

From: TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells

Figure 10

Altered expression of Nrf1 and EMT-specific markers in the Nrf1α−/− cells-derived xenografts.

After scarification of the tumour-bearing mice that had been injected with either HEA157 (Nrf1α−/−) or HepG2 (Nrf1α+/+), the subcutaneous xenograft tissues were obtained and then subjected to the routine histopathological examination by the hematoxylin-eosin staining (HE, (b), followed by immunohistochemical staining with antibodies against Nrf1 (a), the EMT- specific markers E-cadherin (CDH1, (d) and N-cadherin (CDH2, (c), as well as p16/CDKN2A (e). These images shown herein are a representative of at least three independent experiments undertaken on separate occasions that were each performed in duplicate (n = 6). Of note, the xenograft samples were also subjected to quantitative real-time PCR analysis of relevant gene expression (data shown in Fig. S5).

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