Figure 11
Loss of Nrf1α leads to alterations in the deficient cell cycle phases and apoptosis status.
Alterations of either HepG2 (Nrf1α+/+) or HEA157 (Nrf1α−/−) cell cycle (a) and apoptosis (c) were determined by using fluorescence-activated cell sorting (FACS) with different reagents, as described in the section of ‘Materials and methods’. These cell distributions were monitored with the BD Accuri C6 software and also analyzed by the FlowJo 7.6.1 software. The results were calculated as a percentage (%, mean ± S.D.) of cells examined in different phases (b) or apoptosis status (d). Significant increase ($p < 0.05, n = 9) and significant decreases (*p < 0.05, n = 9) in the above alterations resulting from Nrf1α−/− are indicated, relative to the corresponding control values obtained from wild-type Nrf1α+/+ cells. These data shown here are a representative of at least three independent experiments undertaken on separate occasions that were each performed in triplicate. NS represents no significant differences.
