Figure 12
Dysregulated transcriptional expression of distinct genes in Nrf1α−/− cells.
Total RNAs were isolated from HepG2 (Nrf1α+/+) or HEA157 (Nrf1α−/−) cells and then reversely transcribed into the first strand of cDNA. Subsequently, different mRNA levels of distinct genes controlling cell process and behaviour [i.e. cell division cycle (a), apoptosis (b), migration and invasion including the EMT markers (c)] were measured by quantitative real-time PCR. The results were calculated as a fold change (mean ± S.E) of mRNA levels of gene expression in Nrf1α−/− cells and revealed that loss of Nrf1α results in dysregulation of indicated gene transcription with significant decreases (*p < 0.05, n = 9) or significant increases ($p < 0.05, $$p < 0.01, n = 9), relative to their basal mRNA levels of corresponding genes expressed in wild-type Nrf1α+/+ cells (with relevant values being defined as 1). These data shown here are a representative of at least three independent experiments undertaken on separate occasions that were each performed in triplicate.
