Figure 9
Hepatic metastasis of Nrf1α−/− cells-derived subcutaneous tumour xenografts in nude mice.
(a) Small metastatic tumour nodules were seen (directed by arrows) in the livers (but not other organs) in the subcutaneous tumour-bearing mice that had been heterotransplanted with HEA157 (Nrf1α−/−, a1-4), but not with HepG2 (Nrf1α+/+, a5-6). The anatomical section of the liver (a3) was enabled for further observation of hepatic metastasis from the inside (a4), followed by (b) histopathological examination by the routine hematoxylin-eosin staining (HE). The resulting images shown with different magnifications in sizes are a representative of at least three independent experiments undertaken on separate occasions that were each performed in duplicate (n = 6). The obvious areas of hepatic metastatic tumour nodules, along with cancer embolus, were roughly illustrated in the images of the livers in tumour-bearing mice that had been subcutaneously inoculated with Nrf1α−/− (b1-2) rather than Nrf1α+/+ (b3) hepatoma cells.
