Figure 1: Amhr2 is highly expressed in gonadotrope cell lineage and AMH recruits the Smad signaling pathway in LβT2 cells. | Scientific Reports

Figure 1: Amhr2 is highly expressed in gonadotrope cell lineage and AMH recruits the Smad signaling pathway in LβT2 cells.

From: Anti-Müllerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty

Figure 1

Quantification of Amhr2 transcripts in representative cell lines of the different anterior pituitary cell types. Levels of Amhr2 mRNA were measured by Taqman real-time qPCR in the gonadotrope αT3-1 and LβT2, thyrotrope TαT-1, corticotrope AtT20 and somatolactotrope GH3 cell lines. Data are the mean ± SEM of 3 independent cultures. (b) Time course of AMH precursor (AMHp) and cleaved AMH (AMH) effects on Smad1/5/8 phosphorylation in the gonadotrope LβT2 cell line. Cells were treated for 4 or 24 h with 2.5 μg/ml (17.5 nM) AMHp or AMH (concentrations conventionally used in AMH in vitro studies) and Phospho-Smad1/5/8 (P-Smad1/5/8) protein level was evaluated by immunoblotting. Total Smad1 was used as a loading control. (c) No effect of AMH on Smad1/5/8 phosphorylation in the corticotrope AtT20 and the somatotrope GH3 cell lines. Cells were treated for 4 h with 2.5 μg/ml AMH and Phospho-Smad1/5/8 (P-Smad1/5/8) protein level was evaluated as described above. The LβT2 cell line is used as a positive control.

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