Figure 2: Fshb and Inhbb are two target genes of AMH in gonadotrope LβT2 cells. | Scientific Reports

Figure 2: Fshb and Inhbb are two target genes of AMH in gonadotrope LβT2 cells.

From: Anti-Müllerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty

Figure 2

(a) AMH selectively stimulates Fshb and Inhbb expression in LβT2 cells. Cells were stimulated with 2.5 μg/ml AMH for 4 h and transcripts levels were determined by real time qPCR. (b) Time course of AMH precursor and AMH effects on Fshb and inhbb transcripts levels. LβT2 cells were treated for 4 or 24 h with 2.5 μg/ml AMH precursor or AMH. (c) Dose-response of AMH on Fshb transcript levels. LβT2 cells were treated for 4 h with increasing concentrations (0.06 to 10 μg/ml) of AMH precursor or AMH. Fshb mRNA levels were expressed as percentage of control. Data are the mean ± SEM of 3 to 10 experiments. *P ≤ 0.05; **P ≤ 0.001 compared with untreated cells (control).

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