Figure 3: AMH potentiates activin signaling and activin effect on Fshb expression in LβT2 cells. | Scientific Reports

Figure 3: AMH potentiates activin signaling and activin effect on Fshb expression in LβT2 cells.

From: Anti-Müllerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty

Figure 3

LβT2 cells were stimulated for 4 h with 2.5 μg/ml AMH alone or combined with 10 ng/ml activin A or 20 ng/ml BMP2. (a) AMH enhances activin and counteracts BMP2 stimulation of Fshb expression. Fshb and Lhb mRNA levels were determined by real-time qPCR and expressed as the mean ± SEM of at least 3 independent experiments. (b) AMH improves activin coupling to the Smad2/3 pathway. P-Smad2 protein level was evaluated by immunoblotting and normalized with total Smad2. *P ≤ 0.05; **P ≤ 0.01 compared with control cells. a, P ≤ 0.05 between AMH and AMH+activin treatments. b, P ≤ 0.05 between AMH and AMH+BMP2 treatments.

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