Figure 5: Postnatal ontogeny of Amh and Amhr2 expression in anterior pituitary of male and female rats.

Anterior pituitary of male and female Wistar rats were collected at pnd 4, 8, 11, 18, 32 or 70. (a) Ontogeny of Amh and Amhr2 expression in rat pituitary. Transcript levels were measured by Taqman real-time qPCR and expressed as the number of Amh or Amhr2 mRNA copies/μg total RNA. ND: non detectable. (b) Concomitant pituitary expression of gonadotropin subunit transcripts. Levels of Fshb, Lhb and Cga mRNA (arbitrary units) were measured throughout the same developmental period. Each value is a mean ± SEM of 6 to 13 rats. *P ≤ 0.05; **P ≤ 0.01 compared to age-matched male rats.