Figure 1
Reaction phenotypes of T. urartu accessions following Bgt inoculation.
(A) Immune (IM) reaction to powdery mildew. Bgt spore germinated, but neither H2O2 accumulation nor cell death were detected in the leaf area in contact with the spore at 24 hpi. (B) Hypersensitive reaction (HR) to powdery mildew. Bgt spore germinated and elicited a strong H2O2 accumulation (brown precipitates) and cell death at 24 hpi. (C) Susceptible reaction to powdery mildew. Bgt haustorium was observed in the infected host cells at 24 hpi. (D) Bgt hyphal growth in the intercellular space of susceptible T. urartu accessions at 48 hpi. H2O2 accumulation and cell death were detected by staining with 3,3′-diaminobenzidine and trypan blue, respectively. AGT, appressorium germ tube; H, haustorium; Hy, hyphae; PGT, primary germ tube; S, stomata; T, trichome. Bar indicates 25 μm.
