Figure 6
Effects of SB-431542 and GW9662 on the expression of c-fos in the dorsal horn of L4 spinal cord.
(A) c-fos expression in each group as assessed by immunohistochemistry (original magnification × 400). Significantly positive staining of c-fos in the neuron cells was seen in GW9662 group; in contrast, slightly positive staining of c-fos in the neuron cells was observed in the control group; while only very few neuron cells had positive staining of c-fos in the SB-431542 group. The arrows showed the neuron cells with positive staining of c-fos. (B) Protein expression of c-fos in control, SB-431542 and GW9662 groups, respectively. GAPDH was used as the loading control and for band density normalization. (C) The optical density analysis of c-fos protein in three groups. Values are expressed as the mean ± SEM, n = 6 per group *P < 0.05 versus the control group, #P < 0.05 versus the control group.
