Figure 1

Electrophysiological characteristics of human iPSC-derived neurons.

(a) Representative traces showing that a human iPSC-derived neuron displays spontaneous action potentials. (b) Typical traces display action potentials evoked by injecting a series of current steps from −5 pA to +50 pA for 1 s in a human iPSC-derived neuron and the action potentials were eliminated by TTX (0.5 μM). (c) Representative traces showing voltage-gated Na⁺ currents and K⁺ currents elicited by stepping the membrane potentials to a series of potentials from −80 mV to +60 mV for 400 ms in a human iPSC-derived neuron. (d) Spontaneous synaptic activities in the presence of TTX (0.5 μM) in a human iPSC-derived neuron. (e) Representative traces showing mEPSCs under the conditions of untreated (left), treated by AP-5 (100 μM, middle) and treated by AP-5 + CNQX (10 μM, right) in a human iPSC-derived neuron. Inset (far right): averaged mEPSCs before (untreated, black) and during (blue) the application of AP-5 in this cell.