Figure 3

Extracellular Mg²⁺ causes voltage-dependent block on NMDA-evoked currents and synaptic NMDAR currents in human iPSC-derived neurons.

(a) Representative traces showing agonist-evoked NMDAR currents elicited at a series of membrane potentials from −80 mV to +40 mV (in 20 mV increments) in the absence (upper) and presence (bottom) of 2 mM Mg²⁺ in a human iPSC-derived neuron. (b) A plot showing current-voltage relationship of NMDAR currents in the absence and presence of 2 mM Mg²⁺ in human iPSC-derived neurons. n = 3. (c) Left, Typical trace showing mEPSCs recorded at the membrane potentials of −60 mV (bottom) and +60 mV (upper) in presence of extracellular Mg²⁺ at 2 mM in a human iPSC-derived neuron. Right, Averaged mEPSCs in this cell at the membrane potentials of −60 mV and +60 mV.