Figure 4
GluN2B subunit-containing NMDARs are dominant in human iPSC-derived neurons.
(a) Left, Traces showing agonist (50 μM NMDA)-evoked NMDAR currents under control, treated by Ro 25-6981 and treated by NVP-AAM077 + Ro 25-6981 in a human iPSC-derived neuron. Middle, A graph shows NMDAR currents before and after adding Ro 25-6981 and NVP-AAM077 + Ro 25-6981 in 8 recorded human iPSC-derived neurons. Right, Histogram shows NMDAR currents under control, treated with Ro 25-6981 and treated with NVP-AAM077 + Ro 25-6981. n = 8, **P < 0.01, compared with control. (b) Representative traces (left) and graph (right) showing NMDAR currents before and after adding tricine (10 mM). n = 3, P = 0.9 using paired t-test. (c) Typical traces (left) showing NMDAR currents before and after adding 200 nM free Zn2+. Histogram (right) shows the effect of Zn2+ on NMDAR currents in human iPSC-derived neurons. n = 5, *P = 0.014 using paired t-test. (d) Typical traces (left) showing NMDAR currents in a human iPSC-derived neuron before and during TCN 201 (3 μM). Histogram (right) shows the effect of TCN 201 on NMDAR currents in human iPSC-derived neurons. n = 5, P = 0.012 using paired t-test.
