Figure 6
Fyn-interfering peptide Fyn(39–57) inhibits NMDAR currents in human iPSC-derived neurons.
(a) Representative traces show effect of scrambled Fyn(39–57) (30 μg/ml) and Fyn(39–57) (30 μg/ml) on NMDAR currents in human iPSC-derived neurons. (b) A plot showing average NMDAR currents under vehicle alone (n = 6), treated with scrambled Fyn(39–57) (n = 6) and treated with Fyn(39–57) (n = 12). Scrambled Fyn(39–57) and Fyn(39–57) were added via the patch pipette. ***P = 0.0005 (using one-way ANOVA). (c) A plot shows the effect of scrambled Fyn(39–57) and Fyn(39–57) on the current-voltage relationship of NMDAR currents elicited at a series of membrane potentials from −60 mV to + 60 mV (in 40 mV increments) in human iPSC-derived neurons. (d) Representative traces show effect of scrambled Src(40–58) (30 μg/ml) and Src(40–58) (30 μg/ml) on NMDAR currents in human iPSC-derived neurons. A plot showing average NMDAR currents treated with scrambled Src(40–58) (n = 6) and treated with Src(40–58) (n = 9). P > 0.05. (e) Typical traces showing effect of scrambled Fyn(39–57) and Fyn(39–57) on NMDAR currents in human iPSC-derived neurons in the presence of 200 nM free Zn2+. A plot shows average NMDAR currents treated with scrambled Fyn(39–57) and treated with Fyn(39–57) in the presence of 200 nM free Zn2+. n = 8 each, **P = 0.007.
