Figure 2: TGEV infection increases the conversion of LC3-I to LC3-II in ST and PK15 cells.
From: Autophagy Negatively Regulates Transmissible Gastroenteritis Virus Replication
ST cells (a) and PK15 cells (c) were mock-treated or infected with TGEV H165 at MOI of 1. At 6, 12, 24, 30, or 36 h post infection, cells were lysed, separated by reducing SDS-PAGE, and subjected to western blot with the antibodies against LC3, β-actin (loading control), or TGEV N protein as indicated. Densitometry was performed for quantification in ST cells (b) and PK15 cells (d). The ratio of LC3-II to LC3-I (LC3-II/LC3-I) from three independent experiments is expressed as mean ± SD. *p < 0.05. The p value is calculated using Student’s t-test. Gels were run under the same experimental conditions. For better clarity and conciseness of the presentation, cropped blots are shown. The raw uncropped images can be found in the Supplementary Fig. S1.
