Figure 2 | Scientific Reports

Figure 2

From: KCa3.1 mediates dysfunction of tubular autophagy in diabetic kidneys via PI3k/Akt/mTOR signaling pathways

Figure 2

Effect of knockdown KCa3.1 on the expression of LC3 in TGF-β1-exposed HK2 cells.

HK2 cells transfected with scrambled siRNA or KCa3.1 siRNA were exposed to TGF-β1 for 48 h. Immunoblot analysis of LC3 with or without KCa3.1 siRNA transfection in TGF-β1-exposed HK2 cells (a). Quantification of LC3 immunoblot expression in TGF-β1-exposed HK2 cells (b). Immunofluorescence staining of LC3 in TGF-β1-exposed HK2 cells transfected with or without KCa3.1 siRNA (c). Quantification of LC3 immunofluorescence expression in TGF-β1-exposed HK2 cells (d). Results are presented as mean + SEM. **P < 0.01. N = 4. Original magnification: ×600.

Back to article page