Table 1 The targeted genes and design of the dual-sgRNA/Cas9-mediated targeted deletion to create null mutations via deletion or gene replacement.
From: An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design
Gene | Locus | sgRNA1 target sequence with PAM and the expected excision site (↓) | sgRNA2 target sequence with PAM and the expected excision site (↓) | Designed target deletion or replacement region |
|---|---|---|---|---|
AtMIR169a | AT3G13405 | 5′-GAAATAGTTTCTAATTC↓TGGAGG-3′ | 5′-GAGATTTTATGCCCCCA↓AGAAGG-3′ | Chr3:4558557..4559490 (–) |
AtMIR827a | AT3G59884 | 5′-GGATCATCTATTGAAGG↓AACAGG-3′ | 5′-GCAAATCGAAAAGCTTC↓TTAGGG-3′ | Chr3:22122674..22123280 (–) |
AtTFL1 | AT5G03840 | 5′-GCCATTGATAATGGGGA↓GAGTGG-3′ | 5′-GGAAAACTTGTAAGAGG↓AAAAGG-3′ | Chr5:1025496..1025750 (–)* |
