Table 1 Gating properties and toxin inhibition of Kv2.1 and Cav3.1/Kv2.1 chimaeric constructs.

From: Molecular Interactions between Tarantula Toxins and Low-Voltage-Activated Calcium Channels

Construct

V1/2 (mV)

z

Kd (μM)

ProTx-II

PaTx-1

GsAF-I

GsAF-II

Kv2.1

−5.8 ± 1

2.6 ± 0.3

7.4 ± 0.2

17 ± 0.3

36 ± 0.7

23 ± 0.5

DI

4.4 ± 0.9

3.06 ± 0.4

2.2 ± 0.07

2.3 ± 0.2

6.6 ± 0.3

8.5 ± 0.3

E198A

23 ± 2

2.8 ± 0.1

7.7 ± 0.2

5.4 ± 0.2

19 ± 0.6

>50

DII

25 ± 0.7

2.9 ± 0.1

9.2 ± 0.3

16 ± 0.5

11 ± 1

>50

E855A

8 ± 0.5

2.4 ± 0.1

30 ± 0.7

>50

>50

>50

DIII

−1.3 ± 1

2.6 ± 0.1

1.2 ± 0.2

2.1 ± 0.01

2.7 ± 0.2

20 ± 0.2

V1370

−4 ± 1

1.7 ± 0.1

1.3 ± 0.09

2 ± 0.1

2.4 ± 0.2

16 ± 0.6

I1371

21 ± 1

1.3 ± 0.1

1.6 ± 0.2

1.8 ± 0.3

1.6 ± 0.1

7.6 ± 0.1

D1372

11 ± 1

2.1 ± 0.2

9.1 ± 0.4

47 ± 1.1

7.2 ± 0.3

6.4 ± 0.7

I1373

−12 ± 1

2.2 ± 0.1

1.07 ± 0.1

2.1 ± 0.1

2 ± 0.4

7.7 ± 1

L1374

−9 ± 1

1.7 ± 0.1

0.9 ± 0.2

0.9 ± 0.07

2.3 ± 0.03

7.2 ± 0.5

V1375

−14 ± 1

1.7 ± 0.1

1.1 ± 0.1

1.4 ± 0.2

2 ± 0.5

8.8 ± 0.5

S1376

13 ± 1

1.1 ± 0.1

1.1 ± 0.1

0.9 ± 0.06

1.2 ± 0.1

5.4 ± 0.5

M1377

28 ± 1

1.2 ± 0.1

2 ± 0.1

ND

2.8 ± 0.2

11 ± 0.4

V1378

8 ± 2

1.1 ± 0.1

1.3 ± 0.2

1.8 ± 0.1

1.7 ± 0.1

4.7 ± 0.4

S1379

−4 ± 1

1.1 ± 0.1

1.2 ± 0.7

1.3 ± 0.1

2.4 ± 0.2

7.1 ± 0.4

D1380

13 ± 2

1.0 ± 0.1

1.9 ± 0.3

1.8 ± 0.2

2.8 ± 0.2

13 ± 0.5

S1381

25 ± 1

1.1 ± 0.1

1.8 ± 0.1

1.3 ± 0.1

1.3 ± 0.3

7.6 ± 0.2

G1392

26 ± 1

1.5 ± 0.1

1.5 ± 0.3

1.7 ± 0.08

2.2 ± 0.1

8.4 ± 0.4

T1383

7 ± 1

1.3 ± 0.1

1.2 ± 0.02

2.9 ± 0.1

1.9 ± 0.2

6.5 ± 0.4

K1384

5 ± 2

0.9 ± 0.05

1.2 ± 0.1

0.8 ± 0.07

1.3 ± .02

5 ± 0.07

I1385

−7 ± 2

1.1 ± 0.1

1 ± 0.2

2.3 ± 0.1

2.2 ± 0.1

13 ± 0.3

L1386

4 ± 2

0.8 ± 0.05

1.2 ± 0.1

3 ± 0.2

2.4 ± 0.3

19 ± 0.7

G1387

−9 ± 1

1.9 ± 0.1

1.1 ± 0.1

1.7 ± 0.1

2 ± 0.1

11 ± 0.1

M1388

−4 ± 1

1.3 ± 0.1

2.3 ± 0.1

3.2 ± 0.1

2.1 ± 0.1

15 ± 0.5

L1389

−28 ± 2

1.4 ± 0.1

1.8 ± 0.3

3.1 ± 0.07

3.5 ± 0.1

8.6 ± 0.4

DIV

−87 ± 1

3.4 ± 0.3

>50

28 ± 1.2

>50

>50

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