Figure 4: Sirt6 heterozygous mice exhibit increased expression of H60b. | Scientific Reports

Figure 4: Sirt6 heterozygous mice exhibit increased expression of H60b.

From: Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice

Figure 4

(A) The mRNA level of H60b in the aortas was determined (n = 4 each group). The expression level of H60b in aortas from ApoE−/− mice on normal chow diet group normalized to β-Actin was set to 1. P value was obtained by two-way analysis of variance (ANOVA) plus a post hoc analysis using the Bonferroni test. ND: normal chow diet, WD: Western diet. (B) The protein level of H60b in the aortas was determined (n = 4 each group). (C) The H60b protein level in the atherosclerotic plaque was assessed by immunohistochemistry. (D) Quantitative analysis of the expression levels of Sirt6. The total cross lesion area is outlined with green dots and the positive signal was normalized to total cross lesion area. Student’s t test was used to calculate the P value. (E) The mRNA level of inflammation-associated cytokines in aortas of ApoE−/− and Sirt6+/−ApoE−/− fed Western diet for 16 weeks was determined (n = 4 each group). Student’s t test was used to calculate the P value. (*P < 0.05, **P < 0.01, ***P < 0.005).

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