Figure 5: Sirt6 inhibits H60b expression in macrophages. | Scientific Reports

Figure 5: Sirt6 inhibits H60b expression in macrophages.

From: Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice

Figure 5

(A) The mRNA level of H60b expression in macrophages from mice fed with normal chow diet (ND) or Western diet (WD) was determined. The expression level of H60b in macrophages from ApoE−/− mice on normal chow diet group was normalized to β-Actin and was set to 1. P value was obtained by two-way analysis of variance (ANOVA) plus a post hoc analysis using the Bonferroni test. (n = 4 each group) (B) The protein level of H60b was determined similarly with (A) (n = 4 each group). (C) Immunofluorescence and confocal experiments were performed to determine H60b expression in macrophages from ApoE−/− and Sirt6+/−ApoE−/− mice fed Western diet for 16 weeks. (D) Densitometric analysis of the expression level of Sirt6. Student’s t test was used to calculate the P value. (E) Flow cytometry was performed to determine the number of H60b positive cells. The red curve indicates H60b positivity, and the blue curve indicates the negative control. (*P < 0.05, ***P < 0.005).

Back to article page