Figure 7: Sirt6 binds to H60b gene promoter and deacetylates H3K9 and H3K56. | Scientific Reports

Figure 7: Sirt6 binds to H60b gene promoter and deacetylates H3K9 and H3K56.

From: Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice

Figure 7

Peritoneal macrophages were isolated from ApoE−/− and Sirt6+/−ApoE−/− fed with Western diet for 16 weeks. (A) Total H3K9Ac and H3K56Ac levels were determined by western blot. (B–D) ChIP assays were performed to assess Sirt6 binding (B), H3K9Ac level (C), and H3K56Ac level (D) in the promoter of H60b (−158/−53bp). (E–G) Macrophages were infected with Ad-GFP, Ad-Sirt6 or Ad-Sirt6-H133Y, and ChIP assays were performed to assess Sirt6 binding (E), H3K9Ac level (F), and H3K56Ac level (G) in the H60b promoter (−158/−53bp). P value was obtained by two-way analysis of variance (ANOVA) plus a post hoc analysis using the Bonferroni test. (*P < 0.05, **P < 0.01, ***P < 0.005).

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