Figure 6
Vancomycin inhibited the recruitment of myeloid cells into inflamed colon tissue.
(A) Real-time PCR analysis for chemokines in the distal colon at day 10. (B) Flow cytometric analysis of immune cells into the lamina propria of the colon at day 10. Single cell suspensions were stained with FITC-conjugated anti-Gr-1 and eFluor450-conjugated anti-CD11b and analyzed with a FACS Canto flow cytometer as described in the Materials and Methods. (C) Statistical analysis of flow cytometry. (D) Relative presence of bacteria in feces was determined by real-time PCR. DNA samples were extracted from feces at day 24 of AOM/DSS treatment and the amount of total bacteria was determined per unit weight (total bacteria/mg feces). Quantities of each bacterium were normalized to the amount of total bacteria in each sample (n = 4–6). AOM, azoxymethane; DSS, dextran sodium sulfate; FITC, fluorescein isothiocyanate; N, neomycin; n.s., not significant; SEM, standard error of the mean; V, vancomycin; V/N, vancomycin and neomycin. Data represent mean ± SEM (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001.
