Figure 1 | Scientific Reports

Figure 1

From: Two mutations G335D and Q343R within the amyloidogenic core region of TDP-43 influence its aggregation and inclusion formation

Figure 1

Assaying the aggregation abilities of TDP-43 variants by GFP-fusion method.

(a) Schematic representation of the domains in TDP-43 and the genetic mutations in the amyloidogenic core. NT, N-terminal domain; RRM, RNA recognition motif; GRR, glycine-rich region, residues 274–414. TDP-35, the C-terminal fragment of TDP-43, residues 90–414; amyloidogenic core, residues 311–360. H, α-helix; T, turn. (b) The FI/pix ratios of TDP(311–360) and its mutants. FI/pix denotes the ratio of fluorescence intensity to protein amount. The relative GFP-fused protein amount was quantified by recording the grayscale (pixel) of a band in the gel. (c) The FI/pix ratios of TDP-35 and its mutants. (d) The FI/pix ratios of full-length TDP-43 and its mutants. All data from three independent experiments were analyzed statistically by one-way ANOVA and represented as Mean ± SD (n = 3). *p < 0.05; **p < 0.01.

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