Figure 6 | Scientific Reports

Figure 6

From: Two mutations G335D and Q343R within the amyloidogenic core region of TDP-43 influence its aggregation and inclusion formation

Figure 6

Formation of cytoplasmic inclusions of TDP-43 and its mutants.

(a) Immunofluorescence microscopic imaging showing inclusion formation of Myc-tagged TDP-43 and its mutants. STS-treated HeLa cells were stained with a C-terminally Myc-tagged antibody for visualizing TDP-43 species (green) and Hoechst for the nuclei (blue). Scale bar = 10 μm. (b) Quantitation of the cells with inclusion bodies in STS-treated HeLa cells for Myc-tagged TDP-43 and its mutants. Cells were counted and the data were statistically analyzed by one-way ANOVA and shown as Mean ± SD (n = 15–20). ***p < 0.001. (c) Supernatant/pellet fractionation analysis of the deposited species of TDP-43 and its mutants from STS-treated cells. The protein species were detected by Western blotting with an anti-Myc antibody. Sup., Supernatant; Pel., pellet. (d) Quantification of the amounts of the proteolytic TDP-35 species from TDP-43 and its mutants. Acitn was set as a control for relative level. Data were normalized, statistically analyzed by one-way ANOVA and represented as Mean ± SD (n = 3). **p < 0.01; n.s., no significance.

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