Figure 7 | Scientific Reports

Figure 7

From: Two mutations G335D and Q343R within the amyloidogenic core region of TDP-43 influence its aggregation and inclusion formation

Figure 7

Effects of the mutations on the RNA splicing efficiencies of TDP-43 and TDP-35.

(a) Effects of TDP-43 and its mutants on CFTR exon 9 splicing. The CFTR splicing reporter construct GT13T5 was transfected into HEK 293T cells with the indicated FLAG-tagged constructs. Exon 9 + denotes unspliced cDNA fragment, while exon 9- represents spliced cDNA. (b) Quantification of the spliced RT-PCR products of TDP-43 and its mutants. The total intensity of exon 9 + and exon 9- of vector was set as a control. The intensities of band exon 9- of FLAG-TDP-43 and its mutants were compared with the control, respectively. Data were analyzed by one-way ANOVA and represented as Mean ± SD (n = 3). **p < 0.01; n.s., no significance. (c) Effects of TDP-35 and its mutants on CFTR exon 9 splicing. (d) As in (b) quantification of the unspliced RT-PCR products of TDP-35 and its mutants. ***p < 0.001; n.s., no significance.

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