Figure 6
Non-canonical Notch signaling increased the levels of synaptic vesicle proteins.
(a) Induction of Hes5 mRNA expression quantified using qRT-PCR analysis (n = 4–5, means ± SEM, **P < 0.01, ***P < 0.001, compared with EGFP control by Tukey’s test). Expressed proteins in NIH3T3 cells were indicated below the panels. DAPT was used at 10 μM. Schematic depiction of Slm mutation (P269S) in the murine Jagged1 at right. (b) Relative mRNA levels of synaptophysin 1 (syp) and VGLUT1 in the cocultures. (c) Relative protein expression levels of synaptophysin 1 (syp) and VGLUT1 quantified from the band intensities in immunoblot analyses of the coculture (n = 5–17, means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, compared with EGFP control by Steel-Dwass test).
