Figure 7: Inhibition of autophagy by 3-methyladenine diminishes the protective effect for metformin in cisplatin-induced cell apoptosis in NRK-52E cells. | Scientific Reports

Figure 7: Inhibition of autophagy by 3-methyladenine diminishes the protective effect for metformin in cisplatin-induced cell apoptosis in NRK-52E cells.

From: Metformin Protects Against Cisplatin-Induced Tubular Cell Apoptosis and Acute Kidney Injury via AMPKα-regulated Autophagy Induction

Figure 7

(A) Western blot assay showing the LC3-II abundance in NRK-52E cells. NRK-52E cells were incubated with metformin in the absence or presence of 3MA (10 mM) for 12 h. The gels were run under the same experimental conditions. (B) Western blot assay showing caspase 3 cleavage in NRK-52E cells. The gels were run under the same experimental conditions. (C) The graphs showing the semi-quantitative analysis results for the abundance of cleaved caspase 3 protein in NRK-2E cells. *P < 0.05 compared to the cells treated with cisplatin alone (n = 4); #P < 0.05 compared to the cells treated with cisplatin plus metformin (n = 4). (D) Representative micrographs showing the TUNEL staining among different groups as indicated. (E) The graphs showing the quantitative determination of TUNEL-positive cells among different groups. Data are presented as the percentage of the TUNEL-positive cells. *P < 0.05 compared to the cells treated with cisplatin alone (n = 3); #P < 0.05 compared to the cells treated with cisplatin plus metformin (n = 3).

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