Figure 8: Inhibition of AMPKα with siRNA antagonizes the protective effect for metformin in cisplatin-induced cell apoptosis. | Scientific Reports

Figure 8: Inhibition of AMPKα with siRNA antagonizes the protective effect for metformin in cisplatin-induced cell apoptosis.

From: Metformin Protects Against Cisplatin-Induced Tubular Cell Apoptosis and Acute Kidney Injury via AMPKα-regulated Autophagy Induction

Figure 8

(A) Western blot assay showing the downregulation of AMPKα expression in NRK-52E cells transfected with AMPKα siRNA. The gels were run under the same experimental conditions. (B) Western blot assay showing the caspase 3 cleavage after cisplatin treatment in NRK-52E cells. The gels were run under the same experimental conditions. (C) The graphs showing the semi-quantitative analysis of the abundance for cleaved caspase 3 protein in NRK-52E cells within different groups. *P < 0.05 compared to the cells treated with cisplatin alone (n = 3); #P < 0.05 compared to the cells treated with cisplatin plus metformin (n = 3). (D) Representative micrographs showing the results for TUNEL staining among different groups as indicated. (E) The graphs showing the quantitative determination of TUNEL-positive cells among different groups. Data are presented as the percentage of the TUNEL-positive cells. *P < 0.05 compared to the cells treated with cisplatin alone (n = 3); #P < 0.05 compared to the cells treated with cisplatin plus metformin (n = 3).

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