Figure 1
From: Targeted mutagenesis in chicken using CRISPR/Cas9 system
Schematic representation of sgRNAs targeting the OVA and OVM loci.
Exon-intron organization and targeting sequences for the OVA (upper) and OVM (lower) loci are shown. DNA and amino acid sequences are shown in lowercase and uppercase letters, respectively and correspond to the regions indicated by the red arrows. The four sgRNA targeting sites are numbered and represented by black bars above the nucleotide sequence. Adjoining protospacer adjacent motif (PAM) sequences are highlighted in red. The OVMTg1 target site spans the intron 2/exon 3 boundary.
