Table 2 Efficiency of OVM mutation induction in G0 semen and G1 offspring.

From: Targeted mutagenesis in chicken using CRISPR/Cas9 system

Parent (G0)

Number of mutated TA clones from G0 semen (%)a

Number of donor-derived chicks (G1) (%)b

Mutants from donor-derived chicks (%)

#372

12/13 (92%)

33/42 (79%)

19/33 (58%)

#376

15/16 (94%)

29/43 (67%)

14/29 (48%)

#387c

3/20 (15%)*

NT

NT

  1. aThe OVMTg2 region was amplified from rooster semen and individual clones were sequence analyzed. Values represent the percentage of mutated sequences relative to the total number of clones analyzed.
  2. bThe percentage of donor-derived chicks (i.e., black-feathered chicks) was determined relative to the total number of chicks.
  3. cNT, not tested.
  4. *Significantly different compared with #372 and #376, as calculated by a chi-square test (P < 0.001).
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