Table 1 Blastocyst formation from discarded embryos cultured in vitro.

Groups	Female age range	Male age range	No. of embryos cultured	No. of blastocysts formed (% embryos)	No. of high-quality blastocysts formed (% blastocysts)
0PN	22–42	23–43	90	15 (16.7%)^abc	4 (26.7%)^a
1PN	21–43	22–40	28	1 (3.6%)^bd	0
2PN	21–42	22–45	385	87 (22.6%)^ae	33 (37.9%)^a
3PN	21–43	22–44	893	100 (11.2%)^bf	18 (18.0%)^b
≥4PN	21–41	23–40	184	6 (3.3%)^dg	0
2Cell	22–43	24–42	69	44 (63.8%)^h	17 (38.6%)^a
Total			1649	253 (15.3%)	70 (27.7%)

Note: Different superscripts in the same column indicate significant differences (P < 0.05). High-quality blastocysts represent those with grades equal or higher than 3BB (Day 5) or 4BB (Day 6).

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