Figure 2: Top, Annexin V assay to quantify the amount of PS exposed on the outer cell membrane. | Scientific Reports

Figure 2: Top, Annexin V assay to quantify the amount of PS exposed on the outer cell membrane.

From: The rational search for selective anticancer derivatives of the peptide Trichogin GA IV: a multi-technique biophysical approach

Figure 2

Bottom, cholesterol content assay, quantifying the total cellular cholesterol. The error bars are relative to the standard deviation of three repeated experiments. The quantification of the cellular levels of PS in living cells was performed by flow cytometry using Alexa Fluor® 488 Annexin V Kit (Molecular Probes). Total cellular cholesterol was quantified by reverse phase HPLC on cellular homogenates previously treated with cholesterol oxidase and cholesterol esterase.

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