Table 2 Isotropic rotational correlation time of membrane-bound peptides (τ) and percentage of bound peptide obtained from the simulations of the EPR spectra.

From: The rational search for selective anticancer derivatives of the peptide Trichogin GA IV: a multi-technique biophysical approach

Peptide

Correlation time, τ

% membrane-bound peptide

POPC

SUVa

T67

HeLa

HDF

POPC

SUVb

T67

HeLa

HDF

[TOAC1] TG

9 ns

9 ns

12 ns

9 ns

100%

85%

78%

84%

[TOAC1, Lys6] TG

n.a.

10 ns

8 ns

9 ns

100%

96%

89%

97%

[TOAC1, Api4] TG

n.a.

12 ns

12 ns

12 ns

>99%

41%

21%

28%

[TOAC1, Leu4] TG

n.a.

8 ns

9 ns

8 ns

100%

97%

96%

95%

  1. aall spectra in SUV have the same lineshape, only the one for peptide [TOAC1] TG has been simulated. bthe binding percentages between SUV and cells are not directly correlated since the concentrations in the two experiments are not comparable. Principal values of g tensor used in the simulations: gxx = 2.0096, gyy = 2.0064, gzz = 2.0027. Principal values of A tensor: Axx = Ayy = 0.56 mT. The values of Azz are: 3.75 mT for peptides in buffer, 3.27 mT for peptides in SUV, and 3.35 mT for peptides in cells. The variations of Azz are discussed in text.
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