Figure 3

The male-biased EGFP expression in the liver of the pCAG-eGFP Tg mouse line.

(A) The in vivo EGFP fluorescence (Akr1A1^eGFP/+) (a, b) and IHC of EGFP expression (Akr1A1^eGFP/eGFP and Akr1A1^eGFP/+) (c–f) in livers of the male and female Tg mice and the schematic diagram of EGFP expression in the hepatic lobule (g, h). Scale bar: 400 μm; CV: central vein; PT: portal triad. (B) The cell counts of the EGFP+ and EGFP− hepatocytes, which were isolated from male and female Akr1A1^eGFP/+ mice, were analyzed by flow cytometry. Similar results were shown in five animals of each group (n = 5).