Figure 4
The change in expression of EGFP during the development of the liver in male and female Akr1A1eGFP/+ mice.
IHC staining (A) and western blot (B) analysis of the EGFP protein that was expressed in the livers of 1-, 3-, 5- and 7-week-old male and female Tg mice. Scale bar: 100 μm. (C) The quantification of EGFP expression was determined by western blot. β-actin expression was used as an internal control. The bars show the mean ± s.e.m. of five animals per group (n = 5); data were analyzed by one-tailed Student’s t-test; **P < 0.01 vs. the female group at the age of 7 weeks.
