Figure 6
Testosterone masculinizes the expression of EGFP in the Akr1A1eGFP/+ mouse liver.
(A) The male and female Tg mice that underwent gonadectomy at 4 weeks of age and then received either testosterone (2 mg/kg) or 17β-estradiol (50 μg/kg) administration once a day for 2 weeks; the 8-week-old intact female Tg mice were also treated with the same dose of testosterone. After sex hormone administration, the EGFP expression in the mouse liver was analyzed by IHC and in vivo fluorescence imaging. Scale bar: 500 μm. (B) The EGFP expression area of the IHC sections, including those from the intact Tg mice and placebo (cone oil) groups, was quantified ([EGFP area (brown color)/total area] × 100%). The bars show the mean ± s.e.m. of six animals per group (n = 6); data were analyzed by a one-way ANOVA with post hoc comparisons using Duncan’s new multiple range test; *and **represent significant differences (P < 0.05 and P < 0.01, respectively).
