Figure 6
From: Trim69 regulates zebrafish brain development by ap-1 pathway
TRIM69 interacts with c-Jun.
(A,B) HEK-293T cells were transfected with Flag-TRIM69 and HA-c-Jun. After immunoprecipitation with anti-Flag beads, HA-c-Jun was detected by immunoblotting using an anti-HA antibody; after immunoprecipitation with anti-HA beads, Flag-TRIM69 was detected by immunoblotting using an anti-Flag antibody. (C) HeLa cells were co-transfected with Flag-TRIM69 and HA-c-Jun. Subcellular localization of TRIM69 and c-Jun was analyzed by immuno-fluorescence (IF) with anti-Flag and anti-HA antibodies. Nuclei were stained with Hoechst33258.
