Figure 4 | Scientific Reports

Figure 4

From: Conditional Deletion of Fgfr3 in Chondrocytes leads to Osteoarthritis-like Defects in Temporomandibular Joint of Adult Mice

Figure 4

Deletion of Fgfr3 enhances IHH and RUNX2 expression.

Sections of TMJ tissues from 4 month-old mice were analyzed. (A,B) IHH and (C,D) RUNX2 IHC were performed in TMJ cartilage of Cre-negative and Fgfr3 cKO mice. (E,F) Results showing that the expressions of IHH and RUNX2 were significantly enhanced in TMJ cartilage of Fgfr3 cKO mice. Values represent mean ± SD, *p < 0.05 (n = 4 slides per genotype). (G) Primary chondrocytes were isolated form the translucent cartilage tissues of TMJ (arrow) in 4 week-old mice. (H–K) qRT-PCR analysis of mRNA expression in Cre-negative control chondrocytes treated with vehicle (dimethyl sulfoxide), Cre-negative chondrocytes treated with IHH signaling inhibitor (1 μM GDC-0449), Fgfr3-deficient chondrocytes treated with vehicle and Fgfr3-deficient chondrocytes treated with IHH signaling inhibitor. Data are expressed as the normalized fold expression relative to controls. Values represent mean ± SD. *p < 0.05 vs. controls. Scale bar: 50 μm (A–D).

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